Cryopreservation of the Mammary Gland and Improvement on the Culture of the Primary Epithelial Cells in Holstein Dairy Cows
LIN Jie，WANG Xu-rong，WANG Lei，ZHANG Jing-yan，WANG Xue-zhi，MENG Jia-ren，YANG Zhi-qiang*，LI Jian-xi*
(Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province，Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS，Lanzhou 730050，China)
LIN Jie，WANG Xu-rong，WANG Lei，ZHANG Jing-yan，WANG Xue-zhi，MENG Jia-ren，YANG Zhi-qiang，LI Jian-xi. Cryopreservation of the Mammary Gland and Improvement on the Culture of the Primary Epithelial Cells in Holstein Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1067-1074.
［1］STRANDBERG Y，GRAY C，VUOCOLO T，et al.Lipopolysaccharide and lipoteichoic acid induce different innate immune responses in bovine mammary epithelial cells［J］.Cytokine，2005，31(1)：72-86.
［2］BASIRICÒ L，MORERA P，DIPASQUALE D，et al.Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1)［J］.J Dairy Sci，2015，98(10)：7071-7082.
［3］朱佳杰.沼泽型水牛乳腺上皮细胞体外培养体系建立及永生化研究［D］.广西：广西大学，2008.
ZHU J J.Studies on the culture system and immortalization of mammary epithelial cells in swamp buffaloes［D］.Guangxi：Guangxi University，2008.(in Chinese)
［4］李吉霞.牛乳腺上皮细胞永生化及其多能性的研究［D］.杨陵：西北农林科技大学，2010.
LI J X.Immortalization of bovine mammary epithelial cell with multipotency［D］.Yangling：Northwest Agriculture and Forestry University，2010.(in Chinese)
［5］上官陶，王洪梅，冯敏燕，等.表达hTERT及SV40 T永生化奶牛乳腺上皮细胞系的建立［J］.山东农业科学，2013，45(2)：1-4.
SHANGGUAN T，WANG H M，FENG M Y，et al.Establishment of immortalized bovine mammary epithelial cell line expressing hTERT and SV40 T［J］.Shandong Agricultural Sciences，2013，45(2)：1-4.(in Chinese)
［6］代瑞.奶山羊乳腺上皮细胞永生化细胞系的建立［D］.杨陵：西北农林科技大学，2014.
DAI R.The establishment of immortalization of dairy goat mammary epithelial cells［D］.Yangling：Northwest Agriculture and Forestry University，2014.(in Chinese)
［7］刘莉莉．脂肪酸与PPARγ基因互作对奶牛乳腺上皮细胞乳脂肪合成的调节作用及机理［D］.哈尔滨：东北农业大学，2014.
LIU L L.The effect of interaction between fatty acid and PPARγ gene on regulation and mechanism of milk fat synthesis in dairy cow mammary epithelial cells［D］.Harbin：Northeast Agricultural University，2014.(in Chinese)
［8］王立娜.氨基酸与STAT5A基因互作对奶牛乳腺上皮细胞泌乳的调节作用及机理［D］.哈尔滨：东北农业大学，2014.
WANG L N.Effect of interaction between Aminocids and STAT5A on lactation of dairy cow mammary epithelial cells and its mechanism［D］.Harbin：Northeast Agricultural University，2014.(in Chinese)
［9］王治国.奶牛乳腺上皮细胞的体外培养及应用［D］.北京：中国农业科学院，2007.
WANG Z G.In vitro culture and application of bovine mammary epithelial cells［D］.Beijing：Chinese Academy of Agricultural Science，2007.(in Chinese)
［10］WELLNITZ O，KERR D E.Cryopreserved bovine mammary cells to model epithelial response to infection［J］. Vet Immunol Immunopathol，2004，101(3-4)：191-202.
［11］HOU X，LI Q，HUANG T.Microarray analysis of gene expression profiles in the bovine mammary gland during lactation［J］. Sci China Life Sci，2010，53(2)：248-256.
［12］SORG D，DANOWSKI K，KORENKOVA V，et al.Microfluidic high-throughput RT-qPCR measurements of the immune response of primary bovine mammary epithelial cells cultured from milk to mastitis pathogens［J］. Animal，2013，7(5)：799-805.
［13］JEDRZEJCZAK M，SZATKOWSKA I.Bovine mammary epithelial cell cultures for the study of mammary gland functions［J］. In Vitro Cell Dev Biol Anim，2014，50(5)：389-398.
［14］RAUNER G，BARASH I.Xanthosine administration does not affect the proportion of epithelial stem cells in bovine mammary tissue，but has a latent negative effect on cell proliferation［J］. Exp Cell Res，2014，328(1)：186-196.
［15］陈亚冰，符梅，兰道亮，等.牦牛乳腺上皮细胞体外培养及鉴定［J］.畜牧兽医学报，2015，46(1)：69-76.
CHEN Y B，FU M，LAN D L，et al.Culture and identification of yak mammary epithelial cells［J］.Acta Veterinaria et Zootechnica Sinica，2015，46(1)：69-76.(in Chinese)
［16］ZAVIZION B，VAN DUFFELEN M，SCHAEFFER W，et al.Establishment and characterization of a bovine mammary epithelial cell line with unique properties［J］.In Vitro Cell Dev Biol Anim，1996，32(3)：138-148.
［17］PANTSCHENKO A G，BARBER M R，WOODCOCK-MITCHELL J，et al.Establishment and characterization of a caprine mammary myoepithelial cell line (CMMyoEC)［J］. In Vitro Cell Dev Biol Anim，2000，36(6)：351-356.
［18］刘明江.咖啡酸抗LPS诱导的奶牛乳腺上皮细胞炎性损伤作用及其分子机制［D］.北京：中国农业大学，2014.
LIU M J.The protective effect and molecular mechanisms of caffeic acid against inflammation injury of primary bovine mammary epithelial cells induced by lipopolysaccharide［D］.Beijing：China Agricultural University，2014.(in Chinese)
［19］孙苏军，安志兴，彭新荣，等.山羊乳腺组织块冷冻保存试验［J］.西北农林科技大学学报(自然科学版)，2005，33(4)：5-8.
SUN S J，AN Z X，PENG X R，et al.The research of cryopreservation goat mammary tissue［J］.Journal of Northwest A&F University (Natural Science Edition)，2005，33(4)：5-8.(in Chinese)
